【文獻標題】Hydroxycitric Acid Reduced Lipid Droplets Accumulation Via Decreasing Acetyl-Coa Supply and Accelerating Energy Metabolism in Cultured Primary Chicken Hepatocytes

【作者】Longlong Li ,Mengling Peng ,Chongyang Ge, et.al

【作者單位】南京農(nóng)業(yè)大學（Nanjing Agricultural University）

【文獻中引用產(chǎn)品】

雞糖原磷酸化酶(GP)ELISA試劑盒

雞糖原合成酶(GS)ELISA試劑盒

雞葡萄糖激酶(GK)ELISA試劑盒

雞磷酸果糖激酶-1（PFK-1）ELISA試劑盒

雞丙酮酸激酶（PK）ELISA試劑盒

雞丙酮酸脫氫酶E1(PDH E1)ELISA試劑盒

雞檸檬酸合成酶(CS)ELISA試劑盒

雞烏頭酸酶(ACO)ELISA試劑盒

雞磷酸烯醇式丙酮酸羧激酶（PEPCK）ELISA試劑盒

雞ATP-檸檬酸裂解酶(ACLY)ELISA試劑盒

雞NADH脫氫酶ELISA試劑盒

雞ATP合酶（ATP）ELISA試劑盒

【關鍵詞】Hydroxycitric acid ? Acetyl-CoA ? ATP-citrate ? Energy metabolism ? Primary chicken hepatocytes

【DOI】 10.1159/000481564

【出版期刊】《CELLULAR PHYSIOLOGY AND BIOCHEMISTRY》

【產(chǎn)品原文引用】

Measurement of the key glucose metabolic enzyme protein content

Cells were cultured in 6-well plates (2×106 cells per well) and treated with 0, 1, 10 or 50 μM (-)-HCA for 24 h. The cells were harvested and disrupted ultrasonically in ice and then centrifuged at 3000 rpm for 20 min at 4 °C. The supernatants were collected, the protein concentrations of glycogen phosphorylase (GP) (catalog #: E-75175), glycogen synthase (GS) (catalog #: E-75174), glucokinase (GK) (catalog #: E-76111), phosphofructokinase-1 (PFK-1) (catalog #: E-76131), pyruvate kinase (PK) (catalog #: E-76565), pyruvate dehydrogenase (PDH, E1) (catalog #: E-76118), citrate synthase (CS) (catalog #: E-75165), aconitase (ACO) (catalog #: E-75144) and phosphoenolpyruvate carboxykinase (PEPCK) (catalog #: E-75117) were measured using ELISA kits according to the manufactures’ protocols (Shanghai Hengyuan Biological Technology Co., China). The enzyme protein content was normalized to total protein concentration in the sample.